Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols

ISBN-13: 9781071604731 / Angielski / Miękka / 2021 / 451 str.

Paul C. Guest

Spis treści

Part I: Reviews

 

1. The Impact of New Biomarkers and Drug Targets on Age-Related Disorders

            Paul C. Guest

 

2. Vitamin D and Muscle Sarcopenia in Aging

            Behnaz Abiri and Mohammadreza Vafa

 

3. Quantitative Assays of Plasma Apolipoproteins

            Anne Poljak, Mark W. Duncan, Tharusha Jayasena, and Perminder S. Sachdev

 

4. Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice

            Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai

 

Part II: Protocols

 

5. An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models

            Hanél Sadie-Van Gijsen, Liske Kotzé-Hörstmann, and Barbara Huisamen

 

6. Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations

            Yashar Mehrbani Azar, Maria Jacoba Kruger, Dalene de Swardt, Michelle Maartens, Ascentia Mathapelo Seboko, William Frank Ferris, and Mari van de Vyver

 

7. Investigating Alcohol Behavior and Physiology Using Drosophila Melanogaster

            Aliza K. De Nobrega, Kristine V. Luz, Katherine N. Lyons, and Lisa C. Lyons

 

8. Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcript Inhibitors

            Liwei Lang and Yong Teng

 

9. Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System

            Chloe Shay, Liwei Lang, and Yong Teng

 

10. Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells

            Lixia Gao and Yong Teng

 

11. Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines

            Chareeporn Akekawatchai, Sittiruk Roytrakul, Narumon Phaonakrop, Janthima Jaresitthikunchai, and Sarawut Jitrapakdee

 

12. Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. Elegans

            Sumino Yanase

 

13. Clinical Assessment of the NADome as Biomarkers for Healthy Aging

            Tharusha Jayasena, Sonia Bustamante, James Clement, Robert Welschinger, Gideon A. Chaplan, Perminder S. Sachdev, and Nady Braidy

 

14. 2D Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract

            Paul C. Guest

 

15. Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms

            Maximus Berger, G. Paul Amminger, Robyn McDermott, Paul C. Guest, and Zoltán Sarnyai

 

16. Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces Cerevisiae

            Ivan Orlandi and Marina Vai

 

17. Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion is Association with Physiometric and Molecular Biomarkers of Aging

            Paul C. Guest, Hans-Gert Bernstein, Henrik Dobrowolny, Katrin Borucki, Sabine Westphal, and Johann Steiner

 

18. Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue

            Paul C. Guest and Daniel Martins de-Souza

 

19. Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ₁₀ Deficiency

            Robert Heaton, Kate Duberley, and Iain P. Hargreaves

 

20. Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer

            Justin Darcy, Chih-Hao Wang, and Yu-Hua Tseng

 

21. Quantitative Analysis of DNA Methylation by Bisulfite Sequencing

            Vasily V. Ashapkin, Lyudmila I. Kutueva, and Boris F. Vanyushin

 

22. Histomorphometric Analysis of Anti-Aging Properties on Rat Skin

            Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, and Eka Pramyrtha Hestianah

 

23. Integration of qRT-PCR and Immunohistochemical Techniques for mRNA Expression and Localization of m1AChR in the Brain of Aging Rat

            S. Asha Devi and S. Abhijit

 

24. Randomized Study Design to Test Effects of Vitamin D and Omega-3 Fatty Acid Supplementation as Adjuvant Therapy in Colorectal Cancer Patients

            Fatemeh Haidari, Behnaz Abiri, Masood Iravani, Kambiz Ahmadi-Angali, and Mohammadreza Vafa

 

25. Effect of Vitamin D Supplementation on Muscle Strength, Muscle Function, and Body Composition in Vitamin D-Deficient Middle-Aged Women

            Behnaz Abiri, Mohsen Dehghani, and Mohammadreza Vafa

 

26. The Association of Food Intake and Physical Activity with Body Composition, Muscle Strength, and Muscle Function in Postmenopausal Women

            Mohammadreza Vafa, Behnaz Abiri, and Mohsen Dehghani

 

27. Absolute Quantification of Plasma Apolipoproteins for Cardiovascular Disease Risk Prediction

            Betul Ozdemir, Zeliha Selamoglu, and Nady Braidy

 

28. Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases

            Paul C. Guest

 

29. Proteomic Analysis of Brain Tissue from a Chronic Model of Stress Using a Combined 2D Gel Electrophoresis and Mass Spectrometry Approach

            Paul C. Guest

 

30. Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging

            Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de Souza

 

31. Brain Proteomic Analysis on the Effects of the Antidepressant Fluoxetine

            Paul C. Guest

 

32. MK-801 Treatment of Oligodendrocytes as a Cellular Model of Aging

            Paul C. Guest

Blurb

This book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.