Cilia, Part B: Volume 525

ISBN-13: 9780123979445 / Angielski / Twarda / 2013 / 498 str.

Wallace F Marshall

Opis

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers cilia
• Contains chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins

Spis treści

1. Methods for studying ciliary polarity in Xenopus
2. Analysis of signaling pathways in mammalian spermatozoa
3. Analysis of tubulin modification in zebrafish cilia
4. Analysis of microtubule end-binding proteins in cilia
5. Small molecule screens using Chlamydomonas flagella
6. Biochemical and Physiological analysis of axonemal dyneins
7. Analysis of ciliary assembly and function in Planaria
8. Reconstitution of flagellar sliding
9. Comparative genomic analysis of ciliary genes
10. Analysis of ciliogenesis in Tetrahymena
11. Measuring the regulation of dynein activity during flagellar motility
12. Centrosome isolation and proteomics
13. Probing ciliogenesis using micropatterned substrates
14. 3D structural analysis of flagella/cilia by electron cryo-tomography
15. Visualizing IFT in mammalian cilia
16. Ciliogenesis in multiciliated epithelial cells in culture
17. Methods for analysis of PKD-containing urinary exosomes
18. Analysis of Interactions between IFT Proteins
19. Electron microscopy of Intraflagellar Transport
20. Using the SNAP tag to measure ciliary trafficking

O autorze

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.