Chemical Genomics and Proteomics: Reviews and Protocols

ISBN-13: 9781617793486 / Angielski / Twarda / 2011 / 243 str.

Edward D. Zanders

Opis

Chemical genomics technology has been steadily improving, delivering new biological probes and drugs, and the explicit use of the term 'chemical proteomics' has increased with it, as proteins have always been at the heart of this technology. In "Chemical Genomics and Proteomics: Reviews and Protocols," experts in the field present updated reviews of the chemistry of small molecules and their interaction with protein targets as well as detailed protocols that cover different types of ligands, carbohydrates, and lipids. For example, the generation of their protein targets and methods for measuring their interactions is covered. Written in the highly successful "Methods in Molecular Biology " series format, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and up to date, "Chemical Genomics and Proteomics: Reviews and Protocols" aims to provide inspiration to those who wish to use chemical genomics and proteomics in their work and develop this young field into full maturity through the incorporation of the new biological and chemical technologies beginning to emerge here.

Spis treści

Part I: Reviews

 

1. Overview of Chemical Genomics and Proteomics

            Edward D. Zanders

 

2. Exploring Chemical Space: Recent Advances in Chemistry

            Yung-Sing Wong

 

3. In Silico Design of Small Molecules

            Paul H. Bernardo and Joo Chuan Tong

 

4. Surface Plasmon Resonance for Proteomics

            Nico J. de Mol

 

Part II: Protocols

 

5. Biomimetic Affinity Ligands for Immunoglobulins Based on the Multicomponent Ugi Reaction

            Graziella El Khoury, Laura A. Rowe, and Christopher R. Lowe

 

6. Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules

            Hiroshi Takayama, Takashi Moriya, and Naoki Kanoh

 

7. Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids

            Rami N. Hannoush

 

8. Fluorescence Labels in Kinases (FLiK): A High-Throughput Kinase Binding Assay for the Identification of DFG-Out Binding Ligands

            Jeffrey R. Simard and Daniel Rauh

 

9. Electrochemical Aptamer Sensor for Small Molecule Assays

            Xin Liu, Wang Li, Xiahong Xu, Jiang Zhou, and Zhou Nie

 

10. Miniaturized, Microarray-Based Assays for Chemical Proteomic Studies of Protein Function

            Jonathan M. Blackburn, Aubrey Shoko, and Natasha Beeton-Kempen

 

11. Glycan Microarrays

            Xuezheng Song, Jamie Heimburg-Molinaro, David F. Smith, and Richard D. Cummings

 

12. Resolving Bottlenecks for Recombinant Protein Expression in E. coli

            Yoav Peleg and Tamar Unger

 

13. Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System

            Tamar Unger and Yoav Peleg

 

14. Systems for the Cell-Free Synthesis of Proteins

            Lei Kai, Christian Roos, Stefan Haberstock, Davide Proverbio, Yi Ma, Friederike Junge, Mikhail Karbyshev, Zhinan Xu, Volker Dötsch, and Frank Bernhard

 

15. Protein Identification by MALDI-TOF Mass Spectrometry

            Judith Webster and David Oxley