1. Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo
Dong Han and Joseph C. Wu
2. Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations
Tej B. Shrestha and Matthew T. Basel
3. In vivo Epithelial Metabolic Imaging using a Topical Fluorescent Glucose Analog
Paula Villarrea, Rahul Pal, and Gracie Vargas
4. Lipophilic Near Infrared Dyes for in vivo Fluorescent Cell Tracking
Matthew T. Basel
5. Energy-transfer Systems for in vivo Tracking
Raul Neri, Asanka Sajini Yapa, and Stefan H. Bossmann
6. Using Bioengineered Fluorescence for Selective in vivo and ex vivo Tracking of Intestinal Organoids Derived from Human Pluripotent Stem Cells
Hana Leeand Mi-Young Son
7. Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-edited iPSC in Skeletal Muscle
Yue Jin, Yan Shen, Neal L. Weintraub, and Yaoliang Tang
8. Upconversion Fluorescent Nanoprobe for Highly-sensitive in vivo Cell Tracking
Shufang Sheng and Chao Wang
9. Magnetic Resonance Imaging of Single Cells
Madumali Kalubowilage and Stefan H. Bossmann
10. MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles
Wencheng Zhu, Ye Xu, Rongrong Jin, Changqiang Wu, and Hua Ai
11. Protease-activated Sensors for In Vivo Imaging of Cell Populations
Kayla Eschliman and Stefan H. Bossmann
12. Antibody Targeted Magnetic Nanoparticles to Track Immune Cells in Vivo
Kayla Eschliman and Stefan H. Bossmann
13. Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents
Fang Chen and Jesse V. Jokerst
14. Gold Nanoparticles as Computer Tomography Marker for Stem Cell Tracking
Md Nafiujjaman and Taeho Kim
15. Non-Invasive Tracking of Implanted Cells: Superparamagnetic Iron Oxide Nanoparticles as a Long-Term, Multi-Modal Imaging Label
Mughil Sriramvenugopal and Christina A. Pacak
16. Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems that Minimizes Biological Variables
Shili Xu and Harvey R. Herschman
This volume details methods used to track cells in the body and will serve as a reference for preclinical and clinical researchers in the fields of medicine and biomedical science. Chapters guide readers through protocols on bioluminescence imaging, fluorescence imaging, magnetic resonance imaging (MRI), ultrasound, computed tomography, and positron emission spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cell Tracking: Methods and Protocols aims to ensure successful results in the further study of this vital field.