Cell Migration in Three Dimensions

ISBN-13: 9781071628867 / Angielski / Twarda / 2023 / 472 str.

Coert Margadant

Opis

This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes. 

Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1. Cell Migration in Three Dimensions

            Coert Margadant

 

Part I: The Cell Biology of Cell Migration

 

2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis

            Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood

 

3. Probing the ER-Focal Adhesion Link during Cell Migration

            Noemi Antonella Guadagno and Cinzia Progida

 

4. Mapping the Localization of Proteins within Filopodia Using FiloMap

            Guillaume Jacquemet

 

5. Extended Methods for 2D Confinement

            Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel

 

6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63

            Bong Hwan Sung and Alissa M. Weaver

 

7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices

            Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf

 

Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis

 

8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila

            Maik C. Bischoff and Sven Bogdan

 

9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells

            Antonius L. van Boxtel

 

10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure

            Matteo A. Molè, Gabriel L. Galea, and Andrew J. Copp

 

11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila

            Sofia J. Araújo and Marta Llimargas

 

12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland

            Edouard Hannezo and Colinda L.G.J. Scheele

 

13. Analysis of Integrin-Dependent Melanoblast Migration during Development

            Amanda Haage and Guy Tanentzapf

 

Part III: Cell Migration in Cancers and the Tumor Microenvironment

 

14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices

            David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro

 

15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion

            Jian Zhang and Cynthia A. Reinhart-King

 

16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids

            Konstadinos Moissoglu, Stephen J. Lockett, and Stavroula Mili

 

17. Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices

            Daan Smits and Antoine A. Khalil

 

18. Analysis of Collective Migration Patterns within Tumors

            Ralitza Staneva and Andrew G. Clark

 

19. An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation

            Ann L.B. Seynhaeve and Timo L.M. ten Hagen

 

Part IV: Blood Vessel Formation and Interactions

 

20. Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior

            Yuechao Dong, Florian Alonso, Tiya Jahjah, Isabelle Fremaux, and Elisabeth Génot

 

21. Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration

            Maxime Cammeraat, Marko Popovic, Wendy Stam, and Coert Margadant

 

22. Analysis of mRNA Subcellular Distribution in Collective Cell Migration

            Joshua J. Bradbury, Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert

 

23. A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta

            Marta Cherubini and Kristina Haase

 

24. Analysis of Vascular Morphogenesis in Zebrafish

            Marvin Hubert and Wiebke Herzog

 

25. Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging

            Jose M. Gonzalez-Granado, Alberto Del Monte, Laura Piqueras, Vicente Andres, and Cristina Rius