Campylobacter Jejuni: Methods and Protocols

ISBN-13: 9781493965342 / Angielski / Twarda / 2016 / 274 str.

James Butcher; Alain Stintzi

Opis

​​ Campylobacter jejuni (C. jejuni) is often regarded as the one of the most common causes of bacterial gastroenteritis worldwide. The goal of this volume is to highlight key protocols for working with C. jejuni. In particular, chapters aim to highlight recent developments with regards to in vivo models for C. jejuni pathogenesis, different approaches to isolate Campylobacter, and a systems biology approach for studying the effect of all potential Campylobacter gene mutants.Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Campylobacter jejuni: Methods and Protocols encourages existing Campylobacter researchers to employ novel methods to further their own research and also encourages new researchers to include Campylobacter in their future research initiatives.

Spis treści

Campylobacter jejuni Isolation/Enumeration from Environmental Samples.- Recovery of Campylobacter spp. from Food and Environmental Sources.- Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.- Drug Resistance Detection in Campylobacter.- Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni.- Identification of Ligand-receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.- Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.- Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni.- Methods for Initial Characterization of Campylobacter jejuni Bacteriophages.- Methods to Assess the Direct Interaction of C. jejuni with Mucins.- Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.- Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.- Characterisation of Ligand-receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.- Using Galleria Mellonella as an Infection Model for Campylobacter jejuni Pathogenesis.- Mouse Models for Campylobacter jejuni Colonization and Infection.- Metabolomic Analysis of Campylobacter jejuni by Direct Injection Electrospray Ionisation Mass Spectrometry.- Methods for Genome-wide Methylome Profiling of Campylobacter jejuni.- Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni.- Methods for Proteome Analysis of Campylobacter jejuni using 2-D Electrophoresis.- Analyzing Prokaryotic RNA-seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni.- Generation and Screening of an Insertion Sequencing-compatible Mutant Library of Campylobacter jejuni.