Biophysics and the Challenges of Emerging Threats

ISBN-13: 9789048123674 / Angielski / Miękka / 2009 / 179 str.

Joseph D. Puglisi

Opis

Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems 1 3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding 4 6], enzyme kinetics 7, 8], and even protein biosynthesis 1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale 11 13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations 14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen 16 18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases 19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O, blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels."

Spis treści

Preface.- List of Contributors.- A Simple Model for Protein Folding; E.R. Henry, W.A. Eaton.- Complementarity of Hydrophobic/Hydrophilic Properties in Protein Ligand Complexes: A New Tool to Improve Docking Results; T.V. Pyrkov et al.- Structures of Cvnh Family Lectins; A.M. Gronenborn.- Biohysical Approaches to Study DNA Base Flipping; S. Klimasauskas et al.- The Diversity of Nuclear Magnetic Resonance Spectroscopy; C.W. Liu.- Improved Dye Stability in Single-Molecule Fluorescence Experiments; C.E. Aitken et al.- The Evaluation of Isotope Editing and Filtering for Protein-Ligand Interaction Elucidation by NMR; I.M. Robertson et al.- Ribosome: an Ancient Cellular Nano-Machine for Genetic Code Translation; A. Yonath.- Course Abstracts and Posters.- Author Index.-