Baculovirus and Insect Cell Expression Protocols

ISBN-13: 9781493930425 / Angielski / Twarda / 2016 / 508 str.

David W. Murhammer

Opis

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Spis treści

PART I. Introduction

 

1 Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior

                        David W. Murhammer

 

 

PART II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

 

2 Introduction to Baculovirus Molecular Biology

                        Barbara J. Kelly, Linda A. King And Robert D. Possee

 

3 Baculovirus Transfer Vectors

                        Robert D. Possee And Linda A. King

 

4 Recombinant Baculovirus Isolation

                        Linda A. King, Richard Hitchman And Robert D. Possee

 

5 Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System

                        James A. Fornwald, Quinn Lu, Frederick M. Boyce And Robert S. Ames

 

 

PART III. INSECT CELL CULTURE

 

6 Available Lepidopteran Insect Cell Lines

                        Dwight E. Lynn and Robert E. Harrison

 

7 Lepidopteran Insect Cell Line Isolation from Insect Tissue

                        Dwight E. Lynn

 

8 Development Of Serum-Free Media For Lepidopteran Insect Cell Lines

                        Leslie C. L. Chan and Steven Reid

 

9 Routine Maintenance And Storage Of Lepidopteran Insect Cell Lines And Baculoviruses

                        Dwight E. Lynn and Robert L. Harrison

 

 

PART IV. Protein Production with Recombinant Baculoviruses

 

10 Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System

                        Jian-Ping Yang

 

11 Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System

                        Christine M. Thompson, Johnny Montes, Marc G. Aucoin and Amine A. Kamen

 

12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors

                        Sue H. Kadwell and Laurie K. Overton

 

13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae

                        Elena Kovaleva and David C. Davis

 

14 Production Of Virus-Like Particles For Vaccination

                        Christine M. Thompson, Marc G. Aucoin and Amine A. Kamen

 

15 Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells

                        Stephanie Chen

 

 

PART V. Recombinant Protein Production with Transformed Insect Cells

 

16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression

                        Robert L. Harrison and Donald L. Jarvis

 

17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression

                        Marija Backovic and Thomas Krey

 

18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression

                        Robert L. Harrison and Donald L. Jarvis

 

 

PART VI Baculovirus Development and Production for Use as Insecticides

 

19Introduction To The Use Of Baculoviruses As Biological Insecticides

                        Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning

 

20 Baculovirus Insecticide Production In Insect Larvae

                        Nikolai Van Beek and David C. Davis

 

21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses

                        Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning

 

 

PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

 

22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

                        Hsuan-Chen Wu, Hyung Joon Cha and William E. Bentley

 

23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production

                        Hsuan-Chen Wu, Yu-Chen Hu and William E. Bentley

 

24Gene Silencing In Insect Cells Using Rnai

                        Hsuan-Chen Wu, John C. March and William E. Bentley

 

25 Using the Baculovirus/Insect Cell System to Study Apoptosis

                        Nor Chejanovsky

 

26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells

                        Christian Hofmann