Auxins and Cytokinins in Plant Biology: Methods and Protocols

ISBN-13: 9781493968299 / Angielski / Twarda / 2017 / 204 str.

Thomas Dandekar; Muhammad Naseem

Opis

This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.

Spis treści

Preface...
Table of Contents...
Contributing Authors...

 

1. Methodological Advances in Auxin and Cytokinin Biology
   Andrej Hurný and Eva Benková
   
   
2. Analytical Determination of Auxins and Cytokinins
   Petre I. Dobrev, Klára Hoyerová, and Jan Petrášek
   
   
3. Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction
   Jutta Ludwig-Müller, Susann Auer, Sabine Jülke, and Sabine Marschollek
   
   
4. Microbial Manipulation of Auxin and Cytokinins in Plants
   Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
   
   
5. A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling
   Rocío Olmo, Ana Cláudia Silva, Fernando E. Díaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
   
   
6. Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity
   Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
   
   
7. Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK)
   Pallavi Singh and Alok Krishna Sinha
   
   
8. Quantification of Cytokinin Levels and Responses in Abiotic Stresses
   Alfonso Albacete
   
   
9. Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana
   Ruth A. Watson and Cris T. Argueso
   
   
10. Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies
    Martin Schäfer and Stefan Meldau
    
    
11. Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface
    Muhammad Naseem, Shabana Shams, and Thomas Roitsch
    
    
12. Analyzing Cytokinin Responses during Plant-Nematode Interactions
    Florian M.W. Grundler and Shahid Siddique
    
    
13. Examining H₂O₂ Production in Arabidopsis Leaves Upon Challenge by Cytokinin
    Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
    
    
14. A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks
    Meik Kunz, Thomas Dandekar, and Muhammad Naseem
    
    
15. Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots
    Víctor Carrasco Loba, Marta-Marina Pérez Alonso, and Stephan Pollmann
    
    
16. High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation
    Nora Whner, Jörn Herfert, Wolfgang Dröge-Laser, and Christoph Weiste