Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

ISBN-13: 9783319865942 / Angielski / Miękka / 2018 / 191 str.

Marco Rito-Palomares; Jorge Benavides

Spis treści

Chapter 1: General concepts and definitions of Aqueous Two-Phase Systems

Karla Mayolo-Deloisa, Jorge Benavides and Marco Rito-Palomares

Chapter 2: Characterization of Aqueous Two-Phase Systems and their potential new applications

José González-Valdez, Jorge Benavides and Marco Rito-Palomares

Chapter 3: Practical aspects for the development of ATPS-based processes for protein recovery

José González-Valdez and Karla Mayolo-Deloisa

Chapter 4: Aqueous Two-Phase Systems for the recovery of bioparticles

Mirna González-González and Federico Ruiz-Ruiz

Chapter 5: Aqueous Two-Phase Systems strategies for the recovery and partial purification of bioactive low molecular weight compounds

Juan Carlos Sánchez-Rangel, Daniel Jacobo-Velázquez and Jorge Benavides

Chapter 6: Affinity ATPS strategies for the selective fractionation of biomolecules

Federico Ruiz-Ruiz, Jorge Benavides and Marco Rito-Palomares

Chapter 7: Aqueous Two-Phase Systems for the recovery and purification of bioproducts from plants and vegetable tissues

Oscar Aguilar

Chapter 8: Continuous Aqueous Two-Phase System processes

Patricia Vázquez-Villegas and Oscar Aguilar

Chapter 9: Process economics: evaluation of the potential of ATPS as a feasible alternative to traditional fractionation techniques

Mario A. Torres-Acosta, Federico Ruiz-Ruiz, Jorge Benavides and Marco Rito-Palomares

Chapter 10: Perspectives and future trends in Aqueous Two-Phase Systems based technology

Jorge Benavides and Marco Rito-Palomares

O autorze

Marco Rito-Palomares is a professor at Tecnologico de Monterrey in Mexico

Jorge Benavides is a professor at Tecnologico de Monterrey in Mexico

Blurb

This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied.

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area.

Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.