Analytical studies on a standardized extract of Withania somnifera L.

ISBN-13: 9783330343207 / Angielski / Miękka / 2017 / 52 str.

Hafsa Ahmad; Anil Dwivedi; Sarvesh Paliwal

Opis

Withania somnifera Dunal (Ashwagandha) is an Indian medicinal plant with significant pharmacological properties like adaptogenic, antioxidant, hypoglycemic and anti-stroke effects. Several of its chemotypes includes NMITLI-101, NMITLI-118, NMITLI-128. The present work elaborates phytochemical and analytical studies on NMITLI118RT+ a candidate drug under the CSIR umbrella (derived from a new chemotype of W. somnifera). Here we report the development validation and applications of a simple, rapid and reliable isocratic HPLC method for quantification of Withanolide A in NMITLI118RT+ on a CN column utilizing gliclazide as an internal standard which could detect Withanolide A at about 3.8 min and gliclazide (internal standard) at about 4.8 min with a linearity range of 2-20 µg/mL. A second method here reports the development and validation of a simple isocratic method for simultaneous estimation of Withanolide A and Withanone (sesquiterpene lactone markers) established on a C-18; 3µ column which could detect Withanolide A and Withanone at retention times of about 20.7 min and 21.6 min respectively in the linearity range of 2-50 µg/mL for both.

O autorze

The author has pursued her master's and doctoral studies in Pharmacognosy. Her work has extensively dealt with standardization, analytical methodologies and drug delivery systems dealing with herbal candidates and medicinal plants. She is a recipient of several prestigious fellowships and grants and has several research papers, reviews and chapters