Analytical Ultracentrifugation V

ISBN-13: 9783662156346 / Angielski / Miękka / 2013 / 223 str.

H. Colfen

Opis

The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including:
Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies.
In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.

Spis treści

Technical and methodological innovations.- The installation of an eight-cell Schlieren optics multiplexer in a Beckman Optima XLI/XL analytical ultracentrifuge used to measure steep refractive index gradients.- New facilities to improve the properties of Schlieren optics.- Shift interferometry in analytical ultracentrifugation of polymer solutions.- Investigation of the efficiencies of stabilizers for nanoparticles by synthetic boundary crystallization ultracentrifugation.- Innovations in data analysis.- A comparison of evaluation methods for sedimentation velocity data of polymer solutions.- Determination of the particle size distribution of 5–100-nm nanoparticles with the analytical ultracentrifuge: consideration and correction of diffusion effects.- Pseudo synthetic boundary experiments: a new approach to the determination of diffusion coefficients from sedimentation velocity experiments.- Application of the solvent density variation method to sedimentation velocity experiments on biological systems.- Hydrostatic pressure in ultracentrifugation.- The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge.- Allowance for thermodynamic nonideality in the analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants.- Hydrodynamics/Modeling.- Normalized scaling relations as a natural classification of linear macromolecules according to size.- Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules.- Calculation of structural parameters from hydrodynamic data.- Prediction of hydrodynamic parameters from 3D structures.- Synthetic polymers, colloids and supramolecular systems.- Solution properties of supramolecular cobalt coordination arrays.- Working with multidetection in the analytical ultracentrifuge: the benefits of the combination of a refractive index detector and an absorption detector for the analysis of colloidal systems.- Sedimentation velocity analysis of oligomeric enzymes in hydrated reversed micelles of surfactants in organic solvents.- Spectroscopic and hydrodynamic investigations of nonionic and zwitterionic detergents.- Microemulsion polymerization of butyl acrylate and methyl methacrylate.- Sedimentation, translational diffusion, and viscosity of lactosylated polyamidoamine dendrimers.- Biological systems.- A conformation spectrum analysis of the morphological states of myosin S1 in the presence of effectors.- Combining ultracentrifugation with fluorescence to follow the unfolding of modules 16-17 of complement receptor type 1.- Determination of partial molal volumes, and salt and water binding, of highly charged biological macromolecules (tRNA, halophilic protein) in multimolar salt solutions.- Studying membrane proteins in detergent solution by analytical ultracentrifugation: different methods for density matching.- Analytical ultracentrifugation of the nitrogenase of Azotobacter vinelandii under anaerobic conditions.- Combining sedimentation velocity with SEC-MALLS to probe the molecular structure of heterogeneous macromolecular systems that cannot be preparatively separated: application to three rice starches.- Inhomogeneity and conformational parameters of low-substituted carboxymethyl cellulose from analytical ultracentrifugation data.- UV tagging leaves the structural integrity of an arabino-(4-O-methylglucurono)xylan polysaccharide unaffected.- Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide.- Interacting systems and assemblies.- Towards deconvoluting the interaction of the Bacillus subtilis sporulation proteins SinR and SinI using tryptophan analogue incorporated proteins.- Use of the tryptophan analogue 7-azatryptophan to study the interaction of ? N with Escherichia coli RNA polymerase core enzyme.- Nonequilibrium self-association of a cpn60 chaperonin induced by tryptophan mutation.