Acceleration and Improvement of Protein Identification by Mass Spectrometry

ISBN-13: 9781402033186 / Angielski / Twarda / 2005 / 298 str.

Willy Vincent Bienvenut

Opis

At present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'. Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.

Recenzje

"This book is timely. It reviews in a concise form most techniques that should be known by scientists working in a proteomics laboratory or analysing proteins of interest. It first reviews the electrophoretic and chromatographic separation methods. It then summarizes the quantification and identification methods such as immuno-blotting, protein chemistry, peptide fingerprinting or sequencing by fragmentation. Several chapters highlight fascinating developments in the field of mass spectrometry and related techniques. The text shows the reader the perspective of this relatively new field of proteomics.
Finally, the book lists numerous references to critical work done many years ago and unavailable on computer databases. It should therefore be part of every laboratory's library."
(Professor D.F. Hochstrasser)

Spis treści

Preface, Acknowledgements, List of Contributors, 1: Introduction: Proteins analysis using mass spectrometry; 2: Molecular scanner development: Toward clinical molecular scanner for proteome research: Parallel protein chemical processing before and during western-blot; 3: Quantitation during electroblotting step: Enhanced protein recovery after electrotransfer using square wave alternating voltage; 4: Signal traitment and virtual imaging (1/2): A molecular scanner to highly automated research and to display proteome images; 5: Signal traitment and virtual imaging (2/2): Visualization and analysis of molecular scanner peptide mass spectra; 6: Improvement in the peptide mass fingerprint protein identification (1/2): Hydrogen/deuterium exchange for higher specificity of protein identification by peptide mass fingerprinting; 7: Improvement in the peptide mass fingerprint protein identification (2/2): MALDI-MS/MS with high resolution and sensitivity for identification and characterization of proteins; 8: Proteomic and mass spectrometry: Some aspects and recent developments; 9: Conclusions and perspectives; Appendix; Abbreviations used in this book; Abbreviations for usual amino acids and chemical constants; Index.