Lipid-Protein Interactions: Methods and Protocols

ISBN-13: 9781493995141 / Angielski / Miękka / 2020 / 682 str.

Joerg H. Kleinschmidt

Opis

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

Spis treści

Preface...

Table of Contents...

Contributing Authors...

1. Multiscale Modeling and Simulation Approaches to Lipid-Protein Interactions

Roland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine, Jan K. Marzinek, Firdaus Samsudin, Jane R. Allison, Syma Khalid, and Peter J. Bond

2. Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions

Søren B. Nielsen and Daniel E. Otzen

3. Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes

Aleksandra Šakanovič, Vesna Hodnik, and Gregor Anderluh

4. Thermodynamic Analysis of Protein-Lipid Interactions by Isothermal Titration Calorimetry

Musti J. Swamy, Rajeshwer S. Sankhala, and Bhanu Pratap Singh

5. Differential Scanning Calorimetry of Protein-Lipid Interactions

Olga Cañadas and Cristina Casals

6. Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope

K. Tanuj Sapra

7. Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel-Electrophoresis 

Andre Schüßler, Sascha Herwig, and Jörg H. Kleinschmidt

8. Optimized Negative-Staining Protocol for Lipid-Protein Interactions Investigated by Electron Microscopy

Jianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie,  Jinping Li, and Gang Ren

9. Probing Heterogeneous Lipid Interactions with Membrane Proteins using Mass Spectrometry

John W. Patrick and Arthur Laganowsky

10. Protein Microarrays and Liposome: A Method for Studying Lipid-Protein Interactions

Samuel Herianto, Chien-Sheng Chen, and Heng Zhu

11. Structural Investigations of Protein-Lipid Complexes using Neutron Scattering

Luke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, and Jeremy H. Lakey

12. Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment

Koichi Matsuo and Kunihiko Gekko

13. FTIR Analysis of Proteins and Protein-Membrane Interactions

Suren A. Tatulian

14. UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics

DeeAnn K. Asamoto and Judy E. Kim

15. Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching

Gregory A. Caputo and Erwin London

16. Förster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid Selectivity

Luís M. S. Loura, Manuel Prieto, and Fábio Fernandes

17. A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers

Evan L. Taylor, Kumud Raj Poudel, and James A. Brozik

18. Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes

Viktoria Betaneli, Jonas Mücksch, and Petra Schwille

19. Membrane Pore Formation by Peptides Studied by Fluorescence Techniques

Suren A. Tatulian and Nabin Kandel

20. Folding and Insertion of β-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence Spectroscopy

Lisa Gerlach, Omkolsum Gholami, Nicole Schürmann, and Jörg H. Kleinschmidt

21. EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers

Enrica Bordignon, Svetlana Kucher, and Yevhen Polyhach

22. Studying Lipid-Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids

Tibor Páli and Zoltán Kóta

23. Solid-State NMR Approaches to Study Protein Structure and Protein-Lipid Interactions

Christopher Aisenbrey, Evgeniy S. Salnikov, Jesus Raya, Matthias Michalek,  and Burkhard Bechinger

24. Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in Lipid Environment

Ashish Arora

25. Nanodiscs as a New Tool to Examine Lipid-Protein Interactions

Ilia G. Denisov, Mary A. Schuler, and Stephen G. Sligar